Create as many appliqué areas as desired. When stitching out, the appliqué fabric inserts are placed in the reverse order to the order the appliqué areas were created.
If you wish to have overlapping appliqué areas, it is recommended to select the second appliqué method in Appliqué Options, as this will show the changes to the shapes of overlapped areas when you stitch out the embroidery. It is also recommended to play through the embroidery, with Stitch Player, the slider bars, or the Design Player so that you can anticipate the order in which the appliqué areas will be created.